To determine multiple cytokines in a single sample at a single time point, Randox offers a range of unique cytokines arrays allowing complimentary cytokines to be analysed within the same sample.

The combination of highly specific antibodies and advanced chemistries enables cytokines, cytokine receptors and growth factors to be detected simultaneously in a single sample, providing valuable information relating to each cytokine under test and possible associations between cytokines in each sample.

Cytokine Array I

Analyte Intra-Assay Precision (n=20) Intra-Assay Precision (n=20)
  Level 1 %CV Level 2 %CV Level 3 %CV Level 1 %CV Level 2 %CV Level 3 %CV
IL-Ia 9.7 7.7 6.8 10.7 5.9 7.3
IL-1b 8.2 6.3 6.7 13.1 7.3 7.0
IL-2 9.6 5.0 6.3 10.0 7.2 7.6
IL-4 10.7 9.4 4.6 10.0 6.4 7.8
IL-6 12.9 6.1 8.7 15.4 10.4 8.7
IL-8 10.1 7.5 7.9 10.1 8.7 9.9
IL-10 6.3 5.5 6.7 9.4 6.3 6.1
VEGF 7.3 4.6 4.3 13.4 7.6 6.0
IFNγ 12.7 6.2 3.8 13.4 9.8 9.1
EGF 4.6 5.8 7.6 7.4 4.0 7.2
MCP-I 6.8 5.1 4.7 14.7 6.0 5.6
TNFI 10.2 6.0 7.8 13.0 8.1 11.6
Analyte Calibration Range pg/ml Sensitivity pg/ml
IL-Ia 0 - 500 0.8
IL-Ib 0 - 250 1.6
IL-2 0 - 3000 4.8
IL-4 0 - 900 6.6
IL-6 0 - 900 1.2
IL-8 0 - 3000 4.9
IL-10 0 - 1000 1.8
VEGF 0 - 3000 14.6
IFNy 0 - 1500 3.5
EGF 0 - 900 2.9
MCP-I 0 - 1500 13.2
TNFa 0 - 1500 4.4

Accuracy and Precision based on Evidence Investigator

Assay range may vary with batch of calibrators.
Inter-assay precision was determined by assaying 2 replicates of each of three levels of sample in 10 separate assays.
Intra-assay precision was determined by assaying 20 replicates of each of three levels of sample.

Cytokine Array II

Performance data coming soon

Cytokine High Sensitivity Array

Analyte Intra-Assay Precision (n=20) Intra-Assay Precision (n=20)
  Level 1 %CV Level 2 %CV Level 3 %CV Level 1 %CV Level 2 %CV Level 3 %CV
IL-Ia 10.9 9.7 11.4 15.5 8.9 13.1
IL-1b 9.3 8.7 7.4 9.9 11.8 8.7
IL-2 7.8 5.8 6.9 7.9 6.5 8.2
IL-4 8.1 9.5 8.4 11.8 8.6 8.8
IL-6 11.9 9.8 7.8 8.4 7.4 8.4
IL-8 9.4 9.4 7.0 11.1 9.9 9.2
IL-10 6.8 5.6 6.1 6.7 6.5 7.5
VEGF 10.4 10.8 7.3 12.0 7.2 10.7
IFNy 10.1 7.4 7.7 11.4 6.4 7.6
EGF 9.2 9.5 9.6 11.7 8.5 9.6
MCP-I 12.2 8.9 5.8 12.8 10.4 7.2
TNFI 7.1 7.2 12.7 8.6 6.7 7.0
Analyte Calibration Range pg/ml Sensitivity pg/ml
IL-Ia 0 - 225 0.19
IL-Ib 0 - 112.5 0.26
IL-2 0 - 1200 2.97
IL-4 0 - 450 2.12
IL-6 0 - 400 0.12
IL-8 0 - 1450 0.36
IL-10 0 - 450 0.37
VEGF 0 - 1000 3.24
IFNY 0 - 600 0.44
EGF 0 - 450 1.04
MCP-I 0 - 500 3.53
TNFa 0 - 600 0.59

Accuracy and Precision based on Evidence Investigator

Assay range may vary with batch of calibrators.
Inter-assay precision was determined by assaying 2 replicates of each of three levels of sample in 10 separate assays.
Intra-assay precision was determined by assaying 20 replicates of each of three levels of sample.

Cytokine Array III

Analyte Intra-Assay Precision (n=20) Intra-Assay Precision (n=20)
  Level 1 %CV Level 2 %CV Level 3 %CV Level 1 %CV Level 2 %CV Level 3 %CV
IL-5 5.1 8.8 6.5 10.3 9.3 8.1
IL-I5 6.3 8.2 6.2 8.4 6.5 8.6
GMCSF 6.8 6.4 5.2 9.8 9.7 9.7
MIP-1a 9.5 7.8 8.4 11.8 8.6 8.8
Analyte Calibration Range pg/ml Sensitivity pg/ml
IL-5 0 - 1000 0.98
IL-I5 0 - 500 0.74
GMCSF 0 - 500 1.46
MIP-1α 0 - 1500 4.98

Accuracy and Precision based on Evidence Investigator

Assay range may vary with batch of calibrators.
Inter-assay precision was determined by assaying 2 replicates of each of three levels of sample in 10 separate assays.
Intra-assay precision was determined by assaying 20 replicates of each of three levels of sample.

Cytokine Array IV

Analyte Intra-Assay Precision (n=20) Intra-Assay Precision (n=20)
  Level 1 %CV Level 2 %CV Level 3 %CV Level 1 %CV Level 2 %CV Level 3 %CV
sIL-2Rα 7.2 6.6 7.8 6.2 8.7 10.1
sIL-6R 6.7 5.0 7.4 6.1 7.1 8.4
sTNFR I 8.4 8.3 13.9 9.5 12.2 13.1
sTNFR II 9.3 9.2 10.2 9.4 10.3 9.3
MMP-9 10.1 8.7 5.3 8.9 10.2 7.9
Analyte Calibration Range ng/ml Sensitivity ng/ml
sIL-2Rα 0 - 40 0.12
sIL-6R 0 - 400 0.62
GsTNFR I 0 - 25 0.09
sTNFR II 0 - 75 0.20
MMP-9 0 - 1000 3.03

Accuracy and Precision based on Evidence Investigator

Assay range may vary with batch of calibrators.
Inter-assay precision was determined by assaying 2 replicates of each of three levels of sample in 10 separate assays.
Intra-assay precision was determined by assaying 20 replicates of each of three levels of sample.

Cytokine Array V

Analyte Intra-Assay Precision (n=20) Intra-Assay Precision (n=20)
  Level 1 %CV Level 2 %CV Level 3 %CV Level 1 %CV Level 2 %CV Level 3 %CV
IL-3 6.8 6.8 7.8 7.3 10.2 10.7
IL-7 6.3 7.5 8.0 8.7 10.0 9.0
IL-12p70 8.2 6.8 10.0 4.9 7.5 8.2
IL-13 5.5 6.0 8.3 5.9 8.0 10.6
IL-23 7.3 7.2 5.1 6.6 7.3 4.6
Analyte Calibration Range ng/ml Sensitivity ng/ml
IL-3 0 - 2,000 8.78
IL-7 0 - 200 1.11
IL-12p70 0 - 1,000 2.61
IL-13 0 - 2,000 5.23
IL-23 0 - 10,000 13

Accuracy and Precision based on Evidence Investigator

Assay range may vary with batch of calibrators.
Inter-assay precision was determined by assaying 2 replicates of each of three levels of sample in 10 separate assays.
Intra-assay precision was determined by assaying 20 replicates of each of three levels of sample.